Insulin-like growth factor-I and neurogenesis in the adult mammalian brainM.F.Anderson, M.A.I. Aberg, M.Nilsson, P. S. Eriksson (per@neuro.gu.se)
Developmental Brain Research. (2002). V.134. P.115-122. 1, 11-21 (2000)
Developmental Brain Research. (2002). V.134. P.115-122. 1, 11-21 (2000)
Перевод И.Г. Лильп (lilp@mail.ru)
(а) пролиферации,
(б) самоподдержанию,
(в) продукции большого числа дифференцированного, функционального потомства,
(г) регенерации ткани после повреждения и
(д) пластичному использованию этих свойств.
Дочерняя клетка, вступившая на путь дифференцировки называется "transient amplifying cell".
(Рис.1.) | Newly generated cells in the adult human dentate gyrus express a neuronal phenotype. Simultaneous detection of immunofluorescent labels for NeuN (a; scale bar represents 25 mm), BrdU (b) and GFAP (c) for detection of astrocytes and a merge of these signals in x-, y- and z-registration (d) examined by confocal microscopy showed that BrdU-labelled nuclei could specifically co-express NeuN without expressing GFAP (arrows in a-d). In addition to specific BrdU-labelling, some neurons contained non-specific fluorescence in the green and red emission, reflecting their accumulation of lipfuchsin granules (arrowheads in a-d). Red blood cells and endothelial cells, present in several small blood vessels, also emit non-specific green and red fluorescence (small blood vessel indicated by arrowheads, larger blood vessels indicated by asterisks in e-h). The specificity of BrdU-NeuN co-expression in three dimensions is demonstrated by a series of focal planes above (e,f) and below (g,h) the focal plane shown in (d) (arrows indicated the same cell as in d). Adapted from [Eriksson P. et al.1998] and reprinted with permission.
(Рис.2.) | (a) Quantification of BrdU-positive cells in the adult rat hippocampus. The density of BrdU-positive cells (cells / mm3 / sample volume) in the granule cell layer at 1 and 15 days post injection (p.i.) was determined stereologically. IGF-I-treated animals (n =54 at 1day p.i.; n=57 at 15 days p.i.) are hx rats given L-thyroxine, cortisol and IGF-I. Controls (n=55 at 1 day p.i.; n=58 at 15 days p.i.) are hx rats given L-thyroxine and cortisol only. Means±S.E.M. are given. *, P<0.05, **, P<0.01. (b) Percentage of the surviving BrdU-positive cells after 20 days (15 days p.i.) of IGF-I treatment that differentiated into either neurons or astrocytes. Colocalization of BrdU immunoreactivity with cell-specific markers, the granule cell marker Calbindin D28K or the astrocyte marker GFAP, was monitored to determine the phenotype of newborn cells after treatment with IGF-I, when compared to controls in the granule cell layer (n=55 for controls and n=57 for IGF-I-treated animals). Means±S.E.M. are given. *, P<0.05. Adapted from [Aberg M. etal. 2000] and reprinted with permission.
(Рис.1.) | Newly generated cells in the adult human dentate gyrus express a neuronal phenotype. Simultaneous detection of immunofluorescent labels for NeuN (a; scale bar represents 25 mm), BrdU (b) and GFAP (c) for detection of astrocytes and a merge of these signals in x-, y- and z-registration (d) examined by confocal microscopy showed that BrdU-labelled nuclei could specifically co-express NeuN without expressing GFAP (arrows in a-d). In addition to specific BrdU-labelling, some neurons contained non-specific fluorescence in the green and red emission, reflecting their accumulation of lipfuchsin granules (arrowheads in a-d). Red blood cells and endothelial cells, present in several small blood vessels, also emit non-specific green and red fluorescence (small blood vessel indicated by arrowheads, larger blood vessels indicated by asterisks in e-h). The specificity of BrdU-NeuN co-expression in three dimensions is demonstrated by a series of focal planes above (e,f) and below (g,h) the focal plane shown in (d) (arrows indicated the same cell as in d). Adapted from [Eriksson P. et al.1998] and reprinted with permission.
(Рис.2.) | (a) Quantification of BrdU-positive cells in the adult rat hippocampus. The density of BrdU-positive cells (cells / mm3 / sample volume) in the granule cell layer at 1 and 15 days post injection (p.i.) was determined stereologically. IGF-I-treated animals (n =54 at 1day p.i.; n=57 at 15 days p.i.) are hx rats given L-thyroxine, cortisol and IGF-I. Controls (n=55 at 1 day p.i.; n=58 at 15 days p.i.) are hx rats given L-thyroxine and cortisol only. Means±S.E.M. are given. *, P<0.05, **, P<0.01. (b) Percentage of the surviving BrdU-positive cells after 20 days (15 days p.i.) of IGF-I treatment that differentiated into either neurons or astrocytes. Colocalization of BrdU immunoreactivity with cell-specific markers, the granule cell marker Calbindin D28K or the astrocyte marker GFAP, was monitored to determine the phenotype of newborn cells after treatment with IGF-I, when compared to controls in the granule cell layer (n=55 for controls and n=57 for IGF-I-treated animals). Means±S.E.M. are given. *, P<0.05. Adapted from [Aberg M. etal. 2000] and reprinted with permission.
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