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Hippocrates and Galenos, the leading medical doctors of their times and creators of the theory that many diseases are of poisonous origin. Thirteenth century fresco in the crypt of the Domo of Anagni near Rome, Italy. The theoretical and therapeutic concepts of these two masters dominated European medical education and practice until the seventeenth century. The text on the paper in front of Galenos (left) reads "Mundi presentis seres manet ex elementis", meaning "The present world's connection persists on the basis of the elements". The text on the paper in front of Hippocrates (right) reads "Ex his formantur que sunt quecu(m)q(ue) chreantur", meaning "Of these (all) is formed which exists and which will be created". The four basic elements were represented by air, fire, earth and water. Image courtesy of the Art Archive/Anagni Cathedral Italy/Dagli Orti (A).



D

dCAS
  • dCas9-VPR ACTIVATION
      ◊dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy
  • DEAFNESS
  • DRUG-BASED THERAPY
      ◊Generation of Sensory Hair Cells
  • GENOME EDITING
      ◊CRISPR/Cas delivery of genome editing agents
      ◊AAV8 vector enables safe and efficient Cas9 activation
      ◊Photobiomodulation promotes adenoviral gene transduction in auditory cells
  • GENE THERAPY
      ◊Gene Therapy Allows an 11-Year-Old Boy to Hear for the First Time
      ◊AAV-mediated Gene Therapy for Hereditary Deafness: Progress and Perspectives
  • DEGENERATION OF RETINA
  • CELL THERAPY
      ◊Muller cells as a target for retinal therapy
  • DELIVERY SYSTEM
      ◊Exosome-mediated delivery of gene vectors
  • NEW
      ◊A Lactose-Derived CRISPR/Cas9 Delivery System
      ◊Layer-by-Layer technique as a versatile tool for gene delivery applications
  • GENE THERAPY
      ◊CRISPR guided therapies in the dermatology clinic
  • DELIVERY SYSTEM
  • for PROTEINS AND GENES
      ◊ Next Generation Delivery System for Proteins and Genes of Therapeutic Purpose
  • DENTISTRY
  • GENE THERAPY
      ◊ Applications of Gene Therapy in Dentistry
  • DERMAL DIEASES
  • GENE THERAPY
      ◊CRISPR guided therapies in the dermatology clinic
  • DIABETES
  • CELL-BASED THERAPY
      ◊β cells from НЕК cells
      ◊ Engineered Epidermal Progenitor Cells
  • GENE THERAPY
      ◊ Human genetics uncovers MAP3K15 as an obesity-independent therapeutic target for diabetes


  • INSULIN-DEPENDENT DIABETES
      ◊ PARP1 inhibitor of hypoglycaemia
  • SELECTIVE PPARΓ-MODULATING ACTIVITY
      ◊ Telmisartan
  • RESTORATION HIPPOCAMPAL FUNCTION
      ◊cortisol, corticosterone
  • RESTORATION ITS ISLETS
      ◊ Approach
  • TYPE 1
      ◊NKG2D blockade
      ◊Spleen cells
      ◊ Genetic overexpression or pharmacological restoration in hematopoietic stem cells reverses autoimmune diabetes
      ◊ Verapamil and beta cell function in adults with recent-onset type 1 diabetes
      ◊ Vascularization of Tissue Fragments for Efficient Therapeutic Transplantation.
      ◊ Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes
  • TYPE 2
      ◊Allosteric activators of glucokinase
      ◊ A potential therapeutic approach for type 2 diabetes: modulation of carotid sinus nerve activity
      ◊ FGF21 gene therapy as treatment for obesity and insulin resistance

  • DOUBLE KNOCKOUTS
  • METHOD TO ENRICH
      ◊ efficient method to enrich Double Knockouts (DKO) after gene editing

  • DRUG EFFICACY
  • PREDICTION
      ◊ Drug-induced genomic expression profiles
  • PRINCIPLES OF ACTION
      ◊ Drugs action
  • NEW DRUGS
      ◊ siRNA Drug
      ◊ CRISPR/Cas9 from gene editing to drug discovery